Study on Interaction of cefoperazone sodium with ofloxacin in the presence of BSA by fluorescence spectroscopy

Interaction mechanism of cefoperazone sodium (CFP) and ofloxacin (OFX) with bovine serum albumin (BSA) at different temperatures by fluorescence spectrometry method was studied. Results showed that CFP or OFX could quench the fluorescence of BSA. The fluorescence would quench to a larger degree when OFX (or CFP) was added to the system of BSA-CFP (or BSAOFX). The quenching mechanism of the combination for BSA and drugs was a static procedure. The number of binding sites is 1 in various systems. The values of n H were approximately equal to 1. It was proved that the interaction between CFP and OFX simultaneously bound to BSA was weaker and almost no cooperativeness occurred. The site markers competitive experiments indicated that the binding of CFP and OFX to BSA primarily took place in site I. Thermodynamic parameters were also used to identify the force type of drugs with BSA.  2011 Trade Science Inc. INDIA Regular Paper